| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090003 |
| Chromosome: | chromosome_1 |
| Location: | 7071017 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g051050 | FAP225 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGCAGGCCAAGTAGCTCTTGTACTAGCACA |
| Internal bar code: | ATCAATGGGCTTAGGAATTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 99.7748 |
| LEAP-Seq n confirming: | 1329 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGATTCAGAGGCACGAA |
| Suggested primer 2: | CACGCGACCCACATACATAG |