| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090142 |
| Chromosome: | chromosome 17 |
| Location: | 2256261 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713650 | MSH5 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGCTGGCTGGGTCAGCCACCGCATCC |
| Internal bar code: | GTGGCTGGGGACCGGAATCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 564 |
| LEAP-Seq percent confirming: | 79.3522 |
| LEAP-Seq n confirming: | 196 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGTTGAAGAGGCTGAAG |
| Suggested primer 2: | CTTCGCTTACGAAGAGGTGG |