| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090176 |
| Chromosome: | chromosome 6 |
| Location: | 1487570 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259600 | VPS53 | Subunit of GARP complex; (1 of 1) PTHR12820//PTHR12820:SF0 - VACUOLAR SORTING PROTEIN 53 // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCTAGCCTCGCCCCCGCCCCTCCGGC |
| Internal bar code: | TCTAAATCGGTCCGAATACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 99.7215 |
| LEAP-Seq n confirming: | 1432 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCAGCAACGGGTTTAC |
| Suggested primer 2: | TAGCTACGTACCGTACCGGG |