| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090310 |
| Chromosome: | scaffold 24 |
| Location: | 75743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755647 | (1 of 1) K14404 - cleavage and polyadenylation specificity factor subunit 4 (CPSF4, YTH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTTTTCCCCACTCCCCCCTGTCTCGCA |
| Internal bar code: | ACGGCGACGATCGTGGCGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 518 |
| LEAP-Seq percent confirming: | 69.4836 |
| LEAP-Seq n confirming: | 5180 |
| LEAP-Seq n nonconfirming: | 2275 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGCATGAGCGTGATGAG |
| Suggested primer 2: | AGATGCAGAAGCCCAGCTTA |