| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090360 |
| Chromosome: | chromosome 16 |
| Location: | 378430 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693700 | UBC9 | Ubiquitin-conjugating enzyme E2; (1 of 2) K06689 - ubiquitin-conjugating enzyme E2 D/E (UBE2D_E, UBC4, UBC5) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAGTGCGGCGGAAGAAGAGACTAGTCC |
| Internal bar code: | GGAGACAGAACTCTTGAAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 99.6697 |
| LEAP-Seq n confirming: | 5130 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATGTGTTCGGGAGGCT |
| Suggested primer 2: | GTAAAGGCATTGAAGCTCGC |