| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090820 |
| Chromosome: | chromosome 3 |
| Location: | 5043784 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180800 | HDA16 | Histone deacetylase complex protein; (1 of 1) K11644 - paired amphipathic helix protein Sin3a (SIN3A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCAATGCCGCAGCTCACACGCCGCTGT |
| Internal bar code: | GAGCGAGGTGCGTGCGAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 74.5566 |
| LEAP-Seq n confirming: | 3783 |
| LEAP-Seq n nonconfirming: | 1291 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTACACCCCATAATGCC |
| Suggested primer 2: | TTACGTGTGCTCTGGTTTGC |