| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090868 |
| Chromosome: | chromosome 10 |
| Location: | 5715201 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460800 | (1 of 7) 2.7.11.1//2.7.11.17 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCTTGTGTGTATGGGGGTCCCGGGGGA |
| Internal bar code: | CCGGTACAATCGTTCAAGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 435 |
| LEAP-Seq percent confirming: | 65.8586 |
| LEAP-Seq n confirming: | 2608 |
| LEAP-Seq n nonconfirming: | 1352 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGGGATATAAGTTGGGA |
| Suggested primer 2: | GAGCTCAACCGGGTGTATGT |