| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091245 |
| Chromosome: | chromosome 6 |
| Location: | 3854665 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278174 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCGGCCAGCAGTTGCAGGGAGCTGGTG |
| Internal bar code: | ATTGCTCGAACGACGTGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCGTTCACGACTTCAAA |
| Suggested primer 2: | GACACTTGGAGACTCGCACA |