| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091294 |
| Chromosome: | chromosome 7 |
| Location: | 3487626 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCGGGGCCGAGCCTGCGTTTGGAAAGG |
| Internal bar code: | GGAGGTCACGCCTTGGGGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 98.8795 |
| LEAP-Seq n confirming: | 8648 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTTGTTGCTGCTCCACG |
| Suggested primer 2: | CGTGAAGCAAGCATAGTGGA |