| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091299 |
| Chromosome: | chromosome 2 |
| Location: | 3707092 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095200 | (1 of 3) PTHR19211:SF15 - ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGAAACGCGACCCCCTCCACATGGAAT |
| Internal bar code: | ATTAGGATTCGAAGAGTAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 5148 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGTTTGGAGCAGACTGA |
| Suggested primer 2: | GCCCTGCCTACATCAACATT |