| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.092047 |
| Chromosome: | chromosome 17 |
| Location: | 2115900 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712450 | (1 of 2) PF08585 - RecQ mediated genome instability protein (RMI1_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAAACAGCGTAGTACAACGCGGAGCGCT |
| Internal bar code: | ACTATGTCCTGGGTGGTGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 735 |
| LEAP-Seq percent confirming: | 88.4221 |
| LEAP-Seq n confirming: | 5346 |
| LEAP-Seq n nonconfirming: | 700 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTTGCATTGTTTTCGT |
| Suggested primer 2: | CAAGTTTTCTTGCTGAGCCC |