| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.092335 |
| Chromosome: | chromosome 16 |
| Location: | 1265845 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651350 | ATG17 | AuTophaGy related; (1 of 1) PTHR13222//PTHR13222:SF1 - CGTHBA PROTEIN -14 GENE PROTEIN // RB1-INDUCIBLE COILED-COIL PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATGGCTCAGAGCGAAGAATGAGCGGTG |
| Internal bar code: | GCGTCCCCTGAATGTTCTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 99.7345 |
| LEAP-Seq n confirming: | 6387 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGGTACACCCCGCAATC |
| Suggested primer 2: | AGGAGTCAGACGACGCAGAG |