Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.092522 |
Chromosome: | chromosome_4 |
Location: | 2956086 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g224800 | VAMP74,VAM4 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III) | sense | CDS/intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGCAGGCACACGCACACAGATAGAACCAC |
Internal bar code: | GGCCCTGTGGCGGGCCTCGGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1075 |
LEAP-Seq percent confirming: | 99.8003 |
LEAP-Seq n confirming: | 1999 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAAACATGCAACCAAACAC |
Suggested primer 2: | GCCACCACGATCTTCATCTT |