| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.092781 |
| Chromosome: | chromosome_10 |
| Location: | 4462556 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g451600 | antisense | CDS|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTCTTGCCACCGACCTGTACACAATCATT |
| Internal bar code: | GCCGTTATCCCTCAACGTGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 89.4977 |
| LEAP-Seq n confirming: | 1764 |
| LEAP-Seq n nonconfirming: | 207 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAACATGCACAGGGCACA |
| Suggested primer 2: | ACCCACCTGACCACTCTACG |