| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.092824 |
| Chromosome: | chromosome 3 |
| Location: | 4371547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175250 | SQS,SQS1 | Squalene synthase; (1 of 1) 2.5.1.21 - Squalene synthase / Squalene synthetase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGAGCAGCAAGAGCCCACAGCACCGC |
| Internal bar code: | GTGGTGCCGTTAGGCAGTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 65.3846 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTAGAGGTGCTGCTA |
| Suggested primer 2: | CGGCGCCTACTTCTACTACG |