| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.093004 |
| Chromosome: | chromosome 12 |
| Location: | 9252333 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541500 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGTCAGGTGAGTTGGGGTGTGACGAGG |
| Internal bar code: | GTGTACCGTGAGGGGCGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.22 |
| LEAP-Seq n confirming: | 1272 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTGTGGACGTGGAGAAG |
| Suggested primer 2: | TGACCCACTACTGAGGAGCC |