Insertion junction: LMJ.RY0402.093024_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093024
Chromosome:chromosome 1
Location:2318037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g012700 KCN6 (1 of 4) IPR000595//IPR002110//IPR005821//IPR018490//IPR020683 - Cyclic nucleotide-binding domain // Ankyrin repeat // Ion transport domain // Cyclic nucleotide-binding-like // Ankyrin repeat-containing domain; Voltage-gated potassium channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGTCCGCGCCATGCCATCAAGGTCCTCC
Internal bar code:TTGGGTAACAATCTAAAAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:701
LEAP-Seq percent confirming:98.5614
LEAP-Seq n confirming:2672
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCGCGACACTCACTAGA
Suggested primer 2:GCCCTCGCAAGTAGCATAAG