Insertion junction: LMJ.RY0402.093300_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.093300
Chromosome:chromosome_6
Location:2650258
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g269950 EZY21,CDC48,NSG14 Protein involved in ubiquitin-dependent degradation of ER-bound antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TGACGTGTTTGGCATTTGTTCCATGAGAGG
Internal bar code:TTTCGCGCCAGGCAGTCCGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:268
LEAP-Seq percent confirming:99.7835
LEAP-Seq n confirming:2305
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GATGAGCGGAATCCAAGTGT
Suggested primer 2:GTAGGTGGCGACGATTGTTT