| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093581 |
| Chromosome: | chromosome 17 |
| Location: | 2258792 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713650 | MSH5 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATGAGGCATGGCAGGTAAGCTGAAGC |
| Internal bar code: | TTTCAACCTTCGGTGGGTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 29 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACATGGAGAAAGGTGTT |
| Suggested primer 2: | GCCCAAACCAAACCAAACTA |