| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.093639 |
| Chromosome: | chromosome 17 |
| Location: | 2876464 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719500 | AOF9 | (1 of 2) 1.3.99.29 - Phytoene desaturase (zeta-carotene-forming) / 2-step phytoene desaturase; Flavin-containing amine oxidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTGCACACTTTACACTGCAATAGTCA |
| Internal bar code: | TGGGGAAGTTACACCCGCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 195 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCGGGTGATGTGTAACC |
| Suggested primer 2: | CAAATCAGGGTGAGGGAGAA |