Insertion junction: LMJ.RY0402.093717_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.093717
Chromosome:chromosome 12
Location:5965660
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g534700 FKB19 (1 of 1) PTHR10516:SF179 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP19, CHLOROPLASTIC; peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTAAGATGAAATGACCCTCCTCTCACC
Internal bar code:TCAGGACGAATCGATGACACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:201
LEAP-Seq percent confirming:98.4971
LEAP-Seq n confirming:1704
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGTAGCCAACTTTGTCCG
Suggested primer 2:GACACGGTGGTCATTGACTG