| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.093768 |
| Chromosome: | chromosome 3 |
| Location: | 6044605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191250 | LCI34 | Low-CO2-inducible protein 34 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGGGAAGATAGTATCTGAACCGCACGC |
| Internal bar code: | CGGGCTACGACTGGCTGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.5727 |
| LEAP-Seq n confirming: | 12584 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTGTCGGATGACTGAG |
| Suggested primer 2: | GCAGCCATACACCATGACAC |