| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094127 |
| Chromosome: | chromosome 2 |
| Location: | 1224117 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082300 | SUR6 | (1 of 1) PF04935 - Surfeit locus protein 6 (SURF6); Surfeit 6-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTGGCGACAGAATGCGCTCAAAACCA |
| Internal bar code: | ACCGCGTCATGAAGAATTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 226 |
| LEAP-Seq percent confirming: | 43.9369 |
| LEAP-Seq n confirming: | 3482 |
| LEAP-Seq n nonconfirming: | 4443 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTGGCTATGTATGTGTG |
| Suggested primer 2: | AAACCGTAAACATGGCTTGC |