Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.094271 |
Chromosome: | chromosome_3 |
Location: | 3476359 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g167550 | POC3,CEP290 | Centriole proteome protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGCGCCTGCCTCCTCCTCCGCAGGTCAAG |
Internal bar code: | CGAGGATAACGGGGCGCAGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 769 |
LEAP-Seq percent confirming: | 99.2381 |
LEAP-Seq n confirming: | 4168 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGATCGGCATGACTATGGG |
Suggested primer 2: | GTGTGTGGTGATATGGCAGC |