| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094600 |
| Chromosome: | chromosome 4 |
| Location: | 1591807 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213300 | (1 of 88) IPR011333 - POZ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTCCGAGCTGAAGTGATGCCCCGGCAC |
| Internal bar code: | CTCGGGGGCTCGCCCCCCTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 71.6497 |
| LEAP-Seq n confirming: | 2823 |
| LEAP-Seq n nonconfirming: | 1117 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAGTCATGCAAATGTGT |
| Suggested primer 2: | ATCCCGTCTGCAGTACAACC |