| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.094714 |
| Chromosome: | chromosome 17 |
| Location: | 2253601 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713650 | MSH5 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGGGTGTGCGGTTCGTGCGCGTGCA |
| Internal bar code: | ACGTAGGAATGTATGGGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 99.2999 |
| LEAP-Seq n confirming: | 7092 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCTCACTGTATTGCT |
| Suggested primer 2: | AACTTGAGCTGCCCTTCAAA |