| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.094759 |
| Chromosome: | chromosome 9 |
| Location: | 2882271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388372 | RAT2,OPR39 | tscA RNA stabilization factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACATGGTCGGCTTCTTCACACGGCTGG |
| Internal bar code: | CCGCCCAGGGGAAGAGATTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 91.2023 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGACCCCTTGATTCGT |
| Suggested primer 2: | AGTATGGCTTCGTACGGGTG |