| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.094905 |
| Chromosome: | chromosome 2 |
| Location: | 3222130 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095074 | VPS11,ROC12 | (1 of 1) PTHR23323//PTHR23323:SF24 - VACUOLAR MEMBRANE PROTEIN RELATED // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 11 HOMOLOG; Subunit of VPS-C complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGGTACGACACGGGAGAGGGGGAGAGG |
| Internal bar code: | TACACTATCCTAATCCTTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 56.9736 |
| LEAP-Seq n confirming: | 625 |
| LEAP-Seq n nonconfirming: | 472 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTTATTCGTGGGCCCCT |
| Suggested primer 2: | TACCTGGTGGTGGTGAGTGA |