Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.095180 |
Chromosome: | chromosome_3 |
Location: | 4558030 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g176833 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATCATCAGGAGAGGGCGGGGTGAGGGGAG |
Internal bar code: | TAAGAGGAAGTGGTATGCTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 245 |
LEAP-Seq percent confirming: | 90.566 |
LEAP-Seq n confirming: | 96 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAGCTTTGGACTACCCCAC |
Suggested primer 2: | GGGTTAAGGGGTGGTTGATT |