| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.095418 |
| Chromosome: | scaffold 24 |
| Location: | 41096 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755347 | MUT6 | Transgene and transposon silencing 6; (1 of 1) K12815 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX38/PRP16 (DHX38, PRP16) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCAGGTGGCCTCAATCTCCTCCTGGCC |
| Internal bar code: | CGTAAGATATAAATATTTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 52 |
| LEAP-Seq percent confirming: | 99.885 |
| LEAP-Seq n confirming: | 4344 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTACCGAGCTTCCGGTT |
| Suggested primer 2: | CGGTGTCAATGACGTACAGG |