Insertion junction: LMJ.RY0402.095877_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.095877
Chromosome:chromosome 2
Location:5225965
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g106000 (1 of 8) IPR002867 - IBR domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATGACGTAAGTCCGCACCGGTCATCGAC
Internal bar code:TCTGGGCAGTTCGCGTCGTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:421
LEAP-Seq percent confirming:99.1448
LEAP-Seq n confirming:22026
LEAP-Seq n nonconfirming:190
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCTGAGTAGGCTTGGCT
Suggested primer 2:CGTACTCACTTGCGCCTGTA