| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.097208 |
| Chromosome: | chromosome 8 |
| Location: | 4105762 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379650 | TIC20 | 20 kDa translocon at the inner membrane of chloroplasts | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGTTAACAGTTCATCCGACACGCTGA |
| Internal bar code: | GGACTCGTGATCGATCGAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 670 |
| LEAP-Seq percent confirming: | 99.5242 |
| LEAP-Seq n confirming: | 1255 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACATGTTTAGCCGAAAT |
| Suggested primer 2: | CGATTACTAGAATGGGGGCA |