Insertion junction: LMJ.RY0402.097556_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.097556
Chromosome:chromosome 1
Location:867147
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g004550 FAP190 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase; Flagellar Associated Protein 190 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACATCCAATGCATCCCACGTGGCGGGG
Internal bar code:AAGCGTCTCCTGACTCGGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:96.0396
LEAP-Seq n confirming:97
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGTGCACTCATTCCTCC
Suggested primer 2:GTCAAGCGTGTGTCCATGTC