Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.097556 |
Chromosome: | chromosome_1 |
Location: | 867147 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g004550 | FAP190 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCACATCCAATGCATCCCACGTGGCGGGG |
Internal bar code: | AAGCGTCTCCTGACTCGGGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 82 |
LEAP-Seq percent confirming: | 96.0396 |
LEAP-Seq n confirming: | 97 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCATGTGCACTCATTCCTCC |
Suggested primer 2: | GTCAAGCGTGTGTCCATGTC |