Insertion junction: LMJ.RY0402.097605_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.097605
Chromosome:chromosome 15
Location:1779469
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre15.g643503 HKR5,HKR6,HKR56,COP9,COP10 (1 of 1) IPR000104//IPR001054//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006//IPR029730//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily // Archaeal/bacterial/fungal rhodopsin-like // Nucleotide cyclase; Histidine kinase rhodopsin 5/6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGGGGGCACGTGATGAAGGGCCTCGAG
Internal bar code:TCCAAGGGGTTCTGAGGGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:159
LEAP-Seq percent confirming:48.3696
LEAP-Seq n confirming:267
LEAP-Seq n nonconfirming:285
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTCCCACACACTCTCCC
Suggested primer 2:TTCACGTAGTCCACACTGCC