Insertion junction: LMJ.RY0402.098279_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.098279
Chromosome:chromosome 1
Location:4690455
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g032300 GBP1 G-strand telomere DNA binding protein; (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGTGTAGACGACATTGCCGCACTCCCG
Internal bar code:GCCTCGGACGTTGATGCCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:99
LEAP-Seq percent confirming:99.4949
LEAP-Seq n confirming:197
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGCTACTGTCTCCTTGAC
Suggested primer 2:CACGAGGAAGCTTTCGTAGG