| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098434 |
| Chromosome: | chromosome 1 |
| Location: | 5085603 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035350 | (1 of 1) K07512 - mitochondrial trans-2-enoyl-CoA reductase (MECR, NRBF1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACTGAAGCAGGTCTCAGCAACTCAATTC |
| Internal bar code: | ACCCGAGCGGCTTAAGGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 576 |
| LEAP-Seq percent confirming: | 98.9581 |
| LEAP-Seq n confirming: | 4274 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACACCGT |
| Suggested primer 2: | TAACCCCAAAGTGGCTCAAC |