| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.098535 |
| Chromosome: | chromosome 12 |
| Location: | 5258376 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528400 | (1 of 1) PTHR22938:SF0 - ZINC FINGER PROTEIN 598 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGACATAATGGGCACTGTATAGCTGTT |
| Internal bar code: | CACTCGTGTTGCGCTCAAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 272 |
| LEAP-Seq percent confirming: | 98.5948 |
| LEAP-Seq n confirming: | 1263 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTCCTCTGCGTTGAAGG |
| Suggested primer 2: | TCCCTTCTTCGGGGTTAAGT |