| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.098569 |
| Chromosome: | chromosome 13 |
| Location: | 2302291 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579050 | APC1 | (1 of 4) IPR002020 - Citrate synthase; Chlamydomonas specific family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCAGCCCCGACGCCGTGTTGAAGGAGTG |
| Internal bar code: | ACCCGTCGGGTTTAAATTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 928 |
| LEAP-Seq percent confirming: | 99.6126 |
| LEAP-Seq n confirming: | 1800 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTCCAAAGCTTAGACGCC |
| Suggested primer 2: | ACACAAACAGCTTGACAGCG |