| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.098893 |
| Chromosome: | chromosome 17 |
| Location: | 4358582 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731350 | SDR28 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.184//1.1.1.189//1.1.1.197 - Carbonyl reductase (NADPH) / Xenobiotic ketone reductase // Prostaglandin-E(2) 9-reductase / Prostaglandin-E2 9-reductase // 15-hydroxyprostaglandin dehydrogenase (NADP(+)) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCATTGCGGCCAGCTGCGTCAAGAGT |
| Internal bar code: | CCCGAGCCAGCACACGACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 99.0764 |
| LEAP-Seq n confirming: | 9011 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATCAACTTTGCGGGCAC |
| Suggested primer 2: | ATTGGCATGCAGTCTTCACA |