Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.099800 |
Chromosome: | chromosome_7 |
Location: | 1235406 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g321400 | FAP113 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACCAAACTTCGTGGCCCGGATATATATGCG |
Internal bar code: | GGGCTCTGTGGTTTTACATGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 636 |
LEAP-Seq percent confirming: | 98.9428 |
LEAP-Seq n confirming: | 3182 |
LEAP-Seq n nonconfirming: | 34 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGGTAGCGTGATTTCGTT |
Suggested primer 2: | GTCGTGTGGTGTGGTGTTTC |