| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.099881 |
| Chromosome: | chromosome 3 |
| Location: | 4825402 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178900 | PRD1 | (1 of 43) IPR011989 - Armadillo-like helical; putative meiotic recombination protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGTAGGGGAGGCGGCGGCGCCGCCGCC |
| Internal bar code: | GATGGGTTGACTGTTTGTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.6292 |
| LEAP-Seq n confirming: | 5105 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGTGTCATTCGCTGCTG |
| Suggested primer 2: | ATACGGTGTAGCTGGTGGCT |