Insertion junction: LMJ.RY0402.100240_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.100240
Chromosome:chromosome 7
Location:1239650
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTAGATGCCGGTCACCGGATCAAATCCA
Internal bar code:GGGCTTCTCCCCCACAAGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:77
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCGCCAGCAGTAGCTGTA
Suggested primer 2:CTCTCCTCCCATTCCTCCTT