Insertion junction: LMJ.RY0402.100240_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.100240
Chromosome:chromosome 7
Location:1239650
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTGCTTTCTGCTGGAACACGGACTGCGA
Internal bar code:GTGTAAACTAGTATGAGCCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:818
LEAP-Seq percent confirming:99.8094
LEAP-Seq n confirming:3665
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TAGCGCCAGCAGTAGCTGTA
Suggested primer 2:CTCTCCTCCCATTCCTCCTT