| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100330 |
| Chromosome: | chromosome 17 |
| Location: | 1879933 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g710050 | (1 of 6) PF03547 - Membrane transport protein (Mem_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGGAGCGTTCAGCGCAACAACCGCTA |
| Internal bar code: | GAAGAACGCTGGTCGAGTACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 97.751 |
| LEAP-Seq n confirming: | 1782 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTTTGCCATCTTCTGGG |
| Suggested primer 2: | GAATTTGCAGCTGTGGGAAT |