| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.100639 |
| Chromosome: | chromosome 13 |
| Location: | 3273529 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586050 | (1 of 1) PTHR13833:SF49 - NHL REPEAT-CONTAINING PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTAGAACCCCTTAACAGCCCGTCGCCTC |
| Internal bar code: | CTTGCTACACCACTTGTGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 604 |
| LEAP-Seq percent confirming: | 22.0177 |
| LEAP-Seq n confirming: | 1770 |
| LEAP-Seq n nonconfirming: | 6269 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGCATCATAAGTTGCTA |
| Suggested primer 2: | TGAACAGGTTGTCGCTGAAG |