| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.100976 |
| Chromosome: | chromosome_16 |
| Location: | 1161605 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g650450 | FAP219 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTGCTGGTGTTCCAGCCGCTGGGGCCGC |
| Internal bar code: | GCTGGCGCGCAAAACGTGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 81 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGGAGTGCTGACCTTG |
| Suggested primer 2: | CACGCCAGAGCTTATCAACA |