| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101271 |
| Chromosome: | chromosome 16 |
| Location: | 4998648 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685000 | (1 of 3) PTHR13748//PTHR13748:SF43 - COBW-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGCTGGTTGGATGCCTAAGACACAGCA |
| Internal bar code: | GCGGTTTGTAAACAACATTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1044 |
| LEAP-Seq percent confirming: | 99.814 |
| LEAP-Seq n confirming: | 14486 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCCCTTGTAACTCTTGC |
| Suggested primer 2: | GTAAGAGCCGATGCTTTTCG |