| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101353 |
| Chromosome: | chromosome 6 |
| Location: | 7325123 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298850 | LTH6 | LAT2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGATGCATTGCATAAAGGTTTTGCGGA |
| Internal bar code: | AAACTCAGAAGGATCGCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.6749 |
| LEAP-Seq n confirming: | 2146 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCGAGCGCAGTAAGAGAT |
| Suggested primer 2: | TCTTGTCTTGATGTGCCTGG |