Insertion junction: LMJ.RY0402.101489_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.101489
Chromosome:chromosome 1
Location:476010
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTGTGTTGCCAACCATCCACCCGCCCTC
Internal bar code:CTTCCTGGCCTACGGTTCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1000
LEAP-Seq percent confirming:99.8203
LEAP-Seq n confirming:3332
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GATCGTGCTGACAAACCTCA
Suggested primer 2:GCGGATTAAAGCTCAGCAAC