| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101504 |
| Chromosome: | chromosome 11 |
| Location: | 2688548 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g476750 | FNR1 | Ferredoxin-NADP reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGACTGCAGCACTGCTGACTGCTCCGCT |
| Internal bar code: | GGCCTGGCCTGTTGTCGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 141 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACCTGATGGTTTCGTAT |
| Suggested primer 2: | CCACCACAACGTATTTTCCC |